Hammer, M. Schweitzer, D. Leistritz, L. Scibor, M. Donnerhacke, K.H. Strobel, J.: Imaging Spectroscopy at the Human Okular Fundus in vivo. Journal of Biomedical Optics 2 418-425, 1997
Spectroscopic measurement of light which is reflected from biological tissue in vivo is under investigation
for various clinical applications. One special object of investigation using optical methods is the human ocular
fundus.
A fundus reflectometer which enables the simultaneous acquisition of up to 192 spectra arranged in a horizontal
line across the fundus is described. The underlying optical principle of the device is the confocal imaging of
an illuminated narrow slit-like field at the fundus to the entrance slit of a spectrograph. This is imaged by the
grating of the spectrograph onto a two -dimensional CCD chip recording the local distribution of ocular fundus
reflectance spectra within a wavelength range from 400 nm up to 710 nm with a resolution better than 2 nm and a
local resolution of 23 mm in the field dimension of 1.5 mm. The performance of the device was investigated, the
effects of confocal and non-confocal imaging are discussed, and some representative measurements are presented.