Hammer, M. Schweitzer, D. Leistritz, L. Scibor, M. Donnerhacke, K.H. Strobel, J.: Imaging Spectroscopy at the Human Okular Fundus in vivo. Journal of Biomedical Optics 2 418-425, 1997


Spectroscopic measurement of light which is reflected from biological tissue in vivo is under investigation for various clinical applications. One special object of investigation using optical methods is the human ocular fundus.
A fundus reflectometer which enables the simultaneous acquisition of up to 192 spectra arranged in a horizontal line across the fundus is described. The underlying optical principle of the device is the confocal imaging of an illuminated narrow slit-like field at the fundus to the entrance slit of a spectrograph. This is imaged by the grating of the spectrograph onto a two -dimensional CCD chip recording the local distribution of ocular fundus reflectance spectra within a wavelength range from 400 nm up to 710 nm with a resolution better than 2 nm and a local resolution of 23 mm in the field dimension of 1.5 mm. The performance of the device was investigated, the effects of confocal and non-confocal imaging are discussed, and some representative measurements are presented.